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Image Search Results
Journal: Biomedical research (Tokyo, Japan)
Article Title: Estrogen receptor alpha and beta are both involved in the cerebral VEGF/Akt/NO pathway and cerebral angiogenesis in female mice.
doi: 10.2220/biomedres.31.337
Figure Lengend Snippet: Fig. 2 Analysis of phosphorylated Akt (pAkt) (2A, B), and phosphorylated eNOS (2C) shows decreased expression in frontocortical tissues in WT (Con), ERαKO, and ERβKO mice (A–C). Levels of Akt phosphorylated at Ser473 and Thr308 were determined by ELISA. In immunoblotting, β-actin served as loading control. Values are mean ± S.E.
Article Snippet: The following commercially available antibodies were used for Western blot analysis: a) anti-human VEGF rabbit polyclonal antibody (Immuno-Biological Laboratories, Fujioka, Japan), b) anti-human Flt-1 rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), c) anti-human KDR rabbit polyclonal antibody (Santa Cruz Biotechnology), d)
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Control